Triphenyl Tetrazolium Chloride AR

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Triphenyl Tetrazolium Chloride AR Specification

Molecular Formula
C_19H_15N_4Cl

Physical Form
Solid

Shape
Crystalline

Purity
99% (AR Analytical Reagent Grade)

Structural Formula
Ph3C1N4 (Tetrazolium ring with three phenyl groups and a chloride group)

CAS No
298-96-4

EINECS No
206-073-6

Poisonous
Not classified as poisonous (handle with care)

Grade
AR (Analytical Reagent)

Ph Level
Neutral (pH ~7 in aqueous solution)

Melting Point
250C (decomposes)

Other Names
TTC; 2,3,5-Triphenyltetrazolium chloride

Taste
Odourless

Appearance
White to pale pink crystalline powder

Boiling point
Not applicable (decomposes prior to boiling)

HS Code
29339990

Solubility
Slightly soluble in water; soluble in ethanol, methanol, DMSO

Molecular Weight
334.81 g/mol

Smell
Odourless

Hazard Statement
Potential irritant, avoid inhalation and contact with skin and eyes.

Storage Conditions
Store in a tightly closed container in a cool, dry, well-ventilated place, protected from light.

Shelf Life
Minimum 2 years if stored properly.

Stability
Stable under recommended storage conditions.

Packaging Type
Usually supplied in sealed glass bottles or HDPE containers.

Usage/Application
Widely used as a redox indicator in biochemical assays, microbiology (viability staining), enzyme assays, and cellular metabolism studies.

Transport Information
Not classified as dangerous goods under UN regulations.

Typical Assay
99% minimum by HPLC

About Triphenyl Tetrazolium Chloride AR

Triphenyl Tetrazolium Chloride AR is a chemical reagent which is soluble in water. Its melting point is 235 degree C. This chemical contains maximum 0.001% heavy metal, up to 0.2% sulphated ash and maximum 0.001% iron. This chemical has been processed under controlled temperature to keep its properties intact. Quality of Triphenyl Tetrazolium Chloride AR has been verified on the basis of its storage life, composition, purity, chemical stability etc. Customers can avail this chemical at reasonable price from us.

Key Applications and Benefits

Triphenyl Tetrazolium Chloride AR is a highly pure reagent valued for its use as a redox indicator in life sciences. Its main benefits include reliable performance in viability staining for microbiology, efficient enzyme detection, and metabolic activity assessment. Owing to its high assay value, precise results can be obtained in laboratory and research settings, making it an essential resource for academic and industrial research.

Safe Handling and Storage Recommendations

TTC should be stored in a tightly closed container, in a cool, dry, and well-ventilated area protected from light. Proper storage prevents degradation and maintains the quality for at least two years. As a potential irritant, users are advised to avoid inhalation and to prevent contact with skin or eyes by wearing protective equipment during handling.

Packaging and Supply Details

This product is typically packaged in sealed glass bottles or HDPE containers to ensure its integrity during shipping and storage. TTC is not classified as dangerous goods under UN regulations and can be shipped globally by manufacturers, exporters, or traders. Each container is clearly labelled, supporting safe usage and traceability.

FAQs of Triphenyl Tetrazolium Chloride AR:

Q: How should Triphenyl Tetrazolium Chloride AR be stored to preserve its stability and effectiveness?

A: TTC should be kept in a tightly closed container, stored in a cool, dry, and well-ventilated place, and protected from light. These conditions ensure stability and preserve its assay value for up to two years.

Q: What are the main applications of Triphenyl Tetrazolium Chloride AR in laboratories?

A: This compound is widely used as a redox indicator in biochemical assays, viability staining in microbiology, enzyme assays, and in studies assessing cellular metabolism, owing to its precise and reliable indicator properties.

Q: When should personal protective equipment be used with TTC?

A: Personal protective equipment such as gloves, lab coats, and safety goggles should always be used when handling Triphenyl Tetrazolium Chloride AR to avoid inhalation and prevent contact with skin and eyes, as it can be a potential irritant.

Q: Where is Triphenyl Tetrazolium Chloride AR typically supplied from and in what packaging?

A: TTC is commonly supplied by manufacturers, exporters, and traders in India. It is packaged in sealed glass bottles or HDPE containers to maintain product integrity during storage and transport.

Q: What is the process for using TTC in a microbial viability assay?

A: During a microbial viability assay, TTC acts as a redox indicator. Living cells reduce TTC to a red formazan dye, enabling researchers to visually assess cell viability based on the color intensity produced.

Q: What are the benefits of choosing Analytical Reagent (AR) grade TTC?

A: AR grade TTC ensures high purity (99%), which provides accuracy and reliability for sensitive biochemical, enzymatic, and microbial assays. This helps achieve reproducible and trustworthy experimental results.

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